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KMID : 0903519780210030137
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1978 Volume.21 No. 3 p.137 ~ p.143
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Studies on Xanthine Oxidase from Bovine Thyroid Glands
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Abstract
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Xanthine oxidase from bovine thyroid glands was found to contain FAD, molybdenum and iron in a ratio 1:0. 36:1. 6. The molecular weight of the thyroid enzyme was similar to that of the milk enzyme when estimated by gel filtration and polyacrylamide gel electrophoresis. The optimum pH for the enzyme activity was 7.8. The pH of the isoelectric point was determined to be 6.2 by electrofacusing. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis experiment indicated that the enzyme was dissociated into subunits and that the molecular weight for the smallest subunit was 65,000 daltons.
Absorption spectra were dissimilar between milk and thyroid xanthine oxidase.
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